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Canvax ONPG - Galactosidase Assay Kit CA080
PART NO: CX-CA080
ONPG - Galactosidase Assay Kit, optimized, stable & convenient tool to quickly measure the levels of active ß-galactosidase
ONPG B-Galactosidase Assay Kit is an optimized, stable and convenient tool to quickly measure the levels of active ß-galactosidase expressed in cells transfected with plasmids expressing Lac Z. Lac Z is often used reporter gene in experiments transfection because the ß-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. ß-galactosidase performs the hydrolysis of orthonitrophenyl-ß-D-galactopyranoside (ONPG) to the ortho-nitrophenol (ONP). This ONP produces a bright yellow colour that is detected at an absorbance of 420 nm. The concentration of ß-galactosidase is proportional to colour produced.
Main Features:
Proven performance to quantify high expression level of beta-Gal. Very stable: resistant to proteolytic degradation and easily assayed. Convenient for all transfection assays. Versatile: proven performance for cultured cells and tissues. Fast and easy protocol. Cost-effective. Risk-free: product covered by Canvax Quality 100% Guarantee.
Includes: Includes for 500 assays: - ONGP Substrate solution - DTT - Buffer Lysis - Buffer Assay - Buffer Stop - ß-galactosidase enzyme
Applications: Measurement of ß-Galactosidase activity in the lysates of transfected cells.
ONPG B-Galactosidase Assay Kit is an optimized, stable and convenient tool to quickly measure the levels of active ß-galactosidase expressed in cells transfected with plasmids expressing Lac Z. Lac Z is often used reporter gene in experiments transfection because the ß-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. ß-galactosidase performs the hydrolysis of orthonitrophenyl-ß-D-galactopyranoside (ONPG) to the ortho-nitrophenol (ONP). This ONP produces a bright yellow colour that is detected at an absorbance of 420 nm. The concentration of ß-galactosidase is proportional to colour produced.
Main Features:
Proven performance to quantify high expression level of beta-Gal. Very stable: resistant to proteolytic degradation and easily assayed. Convenient for all transfection assays. Versatile: proven performance for cultured cells and tissues. Fast and easy protocol. Cost-effective. Risk-free: product covered by Canvax Quality 100% Guarantee.
Includes: Includes for 500 assays: - ONGP Substrate solution - DTT - Buffer Lysis - Buffer Assay - Buffer Stop - ß-galactosidase enzyme
Applications: Measurement of ß-Galactosidase activity in the lysates of transfected cells.
| Format: 500 assays Shipping conditions: Gel Pack Storage Conditions: 4 ºC | |
| Manufacturer | Canvax |
| Manufacturer P/n | CA080 |