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Home > Laboratory > Life Sciences > Microbiology > Media and Reagents > Dehydrated Culture Media > General Use > Mannitol Salt Agar (M.S.A.) 1062 Condalab

Mannitol Salt Agar (M.S.A.) 1062 Condalab
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Mannitol Salt Agar (M.S.A.) 1062 Condalab

PART NO: CL-1062
Price: €44.01
Out of Stock (Ships in approx. 3 weeks)

Mannitol Salt Agar (MSA) is a selective medium prepared according to the recommendations of Chapman for the isolation of presumptive pathogenic staphylococci. Most of the other bacteria are inhibited due to the high concentration of sodium chloride in this medium. The pancreatic digest of casein, peptic digest of animal tissue and beef extract provide nitrogen, vitamins, minerals, and amino acids essential for growth. Mannitol is the carbohydrate energy source and phenol red is the pH indicator. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent. The degradation of mannitol by bacteria produces acidic products that change the colour of the medium from pink to yellow. Due to its high content of sodium chloride, a heavy inoculum of the material under study can be used.


The European Pharmacopoeia, USP recommends this media for: "Microbiological examination of non-sterile products: Test for specified microorganisms" for the testing of Staphylococcus aureus in products. The addition of 5% Egg Yolk Emulsion (Cat. CL-5152) allows detecting the lipase activity of staphylococci, as well as mannitol fermentation. The high concentration of salt in the medium clears the egg yolk emulsion, and lipase production is detected as a yellow opaque zone around the colonies of staphylococci producing this enzyme. This phenomenon, together with a positive coagulase test, confirms the organism as a pathogenic Staphylococcus.


Formula g/L
Bacteriological agar 15
Beef extract 1
Peptic digest of animal tissue 5
Sodium chloride 75
D-mannitol 10
Pancreatic digest of casein 5
Phenol red 0.025
ManufacturerCondalab
Manufacturer P/n1062

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