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I-Blue MIDI Plasmid Kit 25 preps IBI Scientific IB47181
PART NO: 4AJ-4661764
Price: €301.72
Qty In Stock: 10
The I-Blue Midi Plasmid Kit (25 preps) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
Included in the kit, there is an I-Blue Lysis Buffer (an option color indicator), which helps to prevent common handling errors, making sure that efficient cell lysis and neutralization is achieved.
- Sample Size: 50 – 150ml of cultured bacterial cells
- Operation: anion-exchange resin column, gravity flow
- Expectant Yield: up to 400µg of pure transfection grade plasmid DNA form 100ml cultured bacterial cells
- Elution Volume: 500µl – 2ml
- Operation Time: 80 minutes or less
Sample Packs Available: 4AJ-4661772
Included in the kit, there is an I-Blue Lysis Buffer (an option color indicator), which helps to prevent common handling errors, making sure that efficient cell lysis and neutralization is achieved.
- Sample Size: 50 – 150ml of cultured bacterial cells
- Operation: anion-exchange resin column, gravity flow
- Expectant Yield: up to 400µg of pure transfection grade plasmid DNA form 100ml cultured bacterial cells
- Elution Volume: 500µl – 2ml
- Operation Time: 80 minutes or less
Sample Packs Available: 4AJ-4661772
How It Works?A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. I-Blue lysis buffer is added during the cell re-suspension step, then PM2 buffer is added. After the addition of the PM2 buffer the sample will begin to turn blue during mixing, when the sample is completely blue ,complete lysis has been achieved. Colourless areas or brown clumps indicate incomplete lysis and mixing should continue until the sample is entirely blue. During the neutralization step, PM3 buffer is added and shaking the tube vigorously will begin to turn the sample back to a neutral color. Once the sample is completely colourless, neutralization is complete. Due to the high efficiency of the Midi Column, the entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC, or other toxic reagents. The purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion, and gene gun. FAQWhat is the formulation for the PW buffer in the I-Blue Midi plasmid kit?The exact formulation of the IBI PW buffer is proprietary, but this is QIAGAN’s formulation which will work fine. 1.0 M NaCl, 50 mM MOPS, pH 7.0, 15% Isopropanol | |||||||||||||||||||||||
| Catalogue Number | 4.661 764 | ||||||||||||||||||||||
| Manufacturer | IBI Scientific | ||||||||||||||||||||||
| Manufacturer P/n | IB47181 | ||||||||||||||||||||||