Listeria Agar Base Oxford is a selective medium for Listeria according to the Oxford formula. This medium is recommended for the detection of Listeria monocytogenes from clinical samples and food products. It is used for a direct sample inoculation or it can be used for confirmation after using Listeria Enrichment Broth Base (Cat.CL-1120). All Listeria species hydrolyse the esculin to esculetin. This then reacts with the iron ions producing black colonies and a blackening of the medium. Another advantage of this medium is that peptones and maize starch provides a rich nutrient base for growth, and the addition of Ferric ammonium citrate improves the growth of L. monocytogenes. Lithium chloride is an inhibiting agent, together with the other antibiotics from the supplement, which inhibit the growth of gram-negative bacteria and a large part of Gram-positive ones. Cycloheximide inhibits yeasts.
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